Journal: Microbiome Research Reports

Article Title: The human intestinal bacterium Eggerthella lenta influences gut metabolomes in gnotobiotic mice

doi: 10.20517/mrr.2023.65

Figure Lengend Snippet: E. lenta colonised the caecum of OMM12 mice. (A) Beta-diversity analysis of colonisation groups shown as a multi-dimensional scaling plot of generalised UniFrac distances ( P = 0.001; PERMANOVA); (B) Same as in panel (A), visualised per colonisation/diet groups ( P = 0.001; PERMANOVA); (C) Bacterial composition in caecum of gnotobiotic mice ( 16S rRNA gene amplicon sequencing). Data plotted as dots representing individual mice; black bars indicate median values. B. animalis and A. muris were not detected. Statistics: strains detected in > 50% of the mice in at least one diet group were compared statistically using Mann-Whitney tests with Benjamini-Hochberg adjustment. Stars indicate significant differences between colonisation groups (*adj. P < 0.05; **adj. P < 0.01; including correction within each diet group; n = 11 tests). Different letters indicate significant differences between the corresponding diets for each strain (correction of P -values for each strain individually; n = 12 tests). A previous version of this figure was published in the PhD thesis by co-author Theresa Streidl . OMM12: Oligo-Mouse Microbiota; MM: mice colonised with the mouse synthetic community OMM12; E.L: mice colonised with OMM12 and Coriobacteriia ; CD: control diet; CD-BA: control diet supplemented with 0.2% primary bile acids; HFD: lard-based high-fat diet; HFD-BA: HFD supplemented with 0.2% primary bile acids.

Article Snippet: From week 8 onwards, the diet was changed from autoclaved standard chow (Ssniff GmbH) to synthetic control diet (CD) for two weeks.

Techniques: Amplification, Sequencing, MANN-WHITNEY, Control

Journal: Microbiome Research Reports

Article Title: The human intestinal bacterium Eggerthella lenta influences gut metabolomes in gnotobiotic mice

doi: 10.20517/mrr.2023.65

Figure Lengend Snippet: Colonisation with E. lenta had no significant effects on the host. (A) Weight of the caecum, body, liver, and total WAT as well as blood levels of fasting glucose and cholesterol. Data plotted as dots representing individual mice. Within each group, mice housed in different cages are indicated with empty or filled circles. Black bars show median values. Statistics: Kruskal-Wallis test followed by Dunn’s test for multiple comparisons. Stars indicate significant differences between diets (*adj. P < 0.05; **adj. P < 0.01); (B) PCA of liver proteomes per colonisation group ( P = 0.582; PERMANOVA); (C) Same as in panel (B), shown per diet groups ( P = 0.001; PERMANOVA). Panel (A) of this figure was published in the PhD thesis by co-author Theresa Streidl . WAT: White adipose tissue; PCA: principal component analysis; MM: mice colonised with the mouse synthetic community OMM12; E.L: mice colonised with OMM12 and Coriobacteriia; CD: control diet; CD-BA: control diet supplemented with 0.2% primary bile acids; HFD: lard-based high-fat diet; HFD-BA: HFD supplemented with 0.2% primary bile acids.

Article Snippet: From week 8 onwards, the diet was changed from autoclaved standard chow (Ssniff GmbH) to synthetic control diet (CD) for two weeks.

Techniques: Control

Journal: Microbiome Research Reports

Article Title: The human intestinal bacterium Eggerthella lenta influences gut metabolomes in gnotobiotic mice

doi: 10.20517/mrr.2023.65

Figure Lengend Snippet: Colonisation with E. lenta significantly changed metabolomes in the colon. PCA of all identified metabolite peaks detected in positive ionisation according to (A) colonisation, and (B) diet; (C) Annotated metabolites with significant differences between E.L and MM mice within at least one dietary group as measured in positive ionisation mode. Individual values for the metabolites in grey boxes are shown in panel (D). The symbols after metabolite names indicate those detected in the two ionisation modes and statistically significant in both (*) or only in the specific ionisation mode (#). The strength of significance is shown with a colour gradient (pale yellow to brown). The first coloured column indicates significance (adjusted P -values) according to multi-group comparisons (Kruskal-Wallis with Benjamini-Hochberg adjustment). The remaining columns indicate the results of Dunn’s test for post hoc comparisons between E.L and MM mice per diet. The grey arrows indicate the direction of changes as specified in the figure legend; (D) Peak intensity of the individual metabolites showing significant pairwise comparisons between the two colonisation groups in at least 3 of the 4 diets. Dots represent the values for individual mice; black bars indicate median values; within each group, mice housed in different cages are indicated with empty or filled circles. Statistics: **adj. P < 0.01, ****adj. P < 0.0001, Kruskal-Wallis; for each metabolite, different letters indicate significance between the corresponding groups (Dunn’s test); (E-H) Same as in panels (A-D) for the negative ionisation mode. PCA: Principal component analysis; E.L: mice colonised with OMM12 and Coriobacteriia ; MM: mice colonised with the mouse synthetic community OMM12; CD: control diet; CD-BA: control diet supplemented with 0.2% primary bile acids; HFD: lard-based high-fat diet; HFD-BA: HFD supplemented with 0.2% primary bile acids; OMM12: Oligo-Mouse Microbiota.

Article Snippet: From week 8 onwards, the diet was changed from autoclaved standard chow (Ssniff GmbH) to synthetic control diet (CD) for two weeks.

Techniques: Control